Cellular anatomy of the mouse primary motor cortex.

Muñoz-Castañeda, Rodrigo, Zingg, Brian, Matho, Katherine S, Chen, Xiaoyin, Wang, Quanxin, Foster, Nicholas N, Li, Anan, Narasimhan, Arun, Hirokawa, Karla E, Huo, Bingxing, Bannerjee, Samik, Korobkova, Laura, Park, Chris Sin, Park, Young-Gyun, Bienkowski, Michael S, Chon, Uree, Wheeler, Diek W, Li, Xiangning, Wang, Yun, Naeemi, Maitham, Xie, Peng, Liu, Lijuan, Kelly, Kathleen, An, Xu, Attili, Sarojini M, Bowman, Ian, Bludova, Anastasiia, Cetin, Ali, Ding, Liya, Drewes, Rhonda, D'Orazi, Florence, Elowsky, Corey, Fischer, Stephan, Galbavy, William, Gao, Lei, Gillis, Jesse, Groblewski, Peter A, Gou, Lin, Hahn, Joel D, Hatfield, Joshua T, Hintiryan, Houri, Huang, Junxiang Jason, Kondo, Hideki, Kuang, Xiuli, Lesnar, Philip, Li, Xu, Li, Yaoyao, Lin, Mengkuan, Lo, Darrick, Mizrachi, Judith, Mok, Stephanie, Nicovich, Philip R, Palaniswamy, Ramesh, Palmer, Jason, Qi, Xiaoli, Shen, Elise, Sun, Yu-Chi, Tao, Huizhong W, Wakemen, Wayne, Wang, Yimin, Yao, Shenqin, Yuan, Jing, Zhan, Huiqing, Zhu, Muye, Ng, Lydia, Zhang, Li I, Lim, Byung Kook, Hawrylycz, Michael, Gong, Hui, Gee, James C, Kim, Yongsoo, Chung, Kwanghun, Yang, X William, Peng, Hanchuan, Luo, Qingming, Mitra, Partha P, Zador, Anthony M, Zeng, Hongkui, Ascoli, Giorgio A, Josh Huang, Z, Osten, Pavel, Harris, Julie A, Dong, Hong-Wei (October 2021) Cellular anatomy of the mouse primary motor cortex. Nature, 598 (7879). pp. 159-166. ISSN 0028-0836

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Abstract

An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated and interpreted1. Here, as part of the collaborative Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based anatomical description of one exemplar brain structure, the mouse primary motor cortex, upper limb area (MOp-ul). Using genetic and viral labelling, barcoded anatomy resolved by sequencing, single-neuron reconstruction, whole-brain imaging and cloud-based neuroinformatics tools, we delineated the MOp-ul in 3D and refined its sublaminar organization. We defined around two dozen projection neuron types in the MOp-ul and derived an input-output wiring diagram, which will facilitate future analyses of motor control circuitry across molecular, cellular and system levels. This work provides a roadmap towards a comprehensive cellular-resolution description of mammalian brain architecture.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics organism description > animal bioinformatics > genomics and proteomics > small molecules > Glutamate organism description > animal > mammal organs, tissues, organelles, cell types and functions > tissues types and functions > motor cortex organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organism description > animal > mammal > rodent bioinformatics > genomics and proteomics > small molecules
CSHL Authors:	Matho, Katherine Chen, Xiaoyin Narasimhan, Arun Drewes, Rhonda Elowsky, Corey Fischer, Stephan Galbavy, William Gillis, Jesse Li, Xu Palaniswamy, Ramesh Palmer, Jason Qi, Xiaoli Sun, Yu-Chi Zhan, Huiqing Mitra, Partha P. Zador, Anthony M. Osten, Pavel Munoz-Castaneda, Rodrigo Huo, Bing-Xing Banerjee, Samik Kelly, Kathleen An, Xu Bludova, Anastasiia Hatfield, Joshua Kondo, Hideki Lin, Meng Kuan Mizrachi, Judith Huang, Z. Josh
Communities:	CSHL labs > Gillis Lab CSHL labs > Huang lab CSHL labs > Mitra lab CSHL labs > Osten lab CSHL labs > Zador lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	October 2021
Date Deposited:	13 Oct 2021 14:38
Last Modified:	25 Jan 2024 15:37
PMCID:	PMC8494646
URI:	https://repository.cshl.edu/id/eprint/40384

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