Naguib, A., Bencze, G., Engle, D. D., Chio, II, Herzka, T., Watrud, K., Bencze, S., Tuveson, D. A., Pappin, D. J., Trotman, L. C.
(December 2014)
P53 Mutations Change Phosphatidylinositol Acyl Chain Composition.
Cell Reports, 10 (1).
pp. 8-19.
ISSN 2211-1247
Abstract
Phosphatidylinositol phosphate (PIP) second messengers relay extracellular growth cues through the phosphorylation status of the inositol sugar, a signal transduction system that is deregulated in cancer. In stark contrast to PIP inositol head-group phosphorylation, changes in phosphatidylinositol (PI) lipid acyl chains in cancer have remained ill-defined. Here, we apply a mass-spectrometry-based method capable of unbiased high-throughput identification and quantification of cellular PI acyl chain composition. Using this approach, we find that PI lipid chains represent a cell-specific fingerprint and are unperturbed by serum-mediated signaling in contrast to the inositol head group. We find that mutation of Trp53 results in PIs containing reduced-length fatty acid moieties. Our results suggest that the anchoring tails of lipid second messengers form an additional layer of PIP signaling in cancer that operates independently of PTEN/PI3-kinase activity but is instead linked to p53.
| Item Type: |
Paper
|
| Subjects: |
diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53 |
| CSHL Authors: |
Bencze, Gyula
Engle, Dannielle
Herzka, Tali
Naguib, Adam
Pappin, Darryl J.
Trotman, Lloyd C.
Tuveson, David A.
Bencze, Szilvia
Chio, Iok In
Watrud, Kaitlin
|
| Communities: |
CSHL Cancer Center Program > Signal Transduction
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Animal Tissue and Imaging Service
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL Cancer Center Shared Resources > Mass Spectrometry Service
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL labs > Pappin lab
CSHL labs > Trotman lab
CSHL labs > Tuveson lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service |
| Depositing User: |
Matt Covey
|
| Date: |
24 December 2014 |
| Date Deposited: |
05 Jan 2015 16:47 |
| Last Modified: |
16 Jul 2021 20:02 |
| PMCID: |
PMC4287966 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/31020 |
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