Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

Stolarov, J., Chang, K., Reiner, A., Rodgers, L., Hannon, G. J., Wigler, M. H., Mittal, V. (November 2001) Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor. Proceedings of the National Academy of Sciences of the United States of America, 98 (23). pp. 13043-13048. ISSN 0027-8424

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URL: https://www.ncbi.nlm.nih.gov/pubmed/11687610

DOI: 10.1073/pnas.221450598

Abstract

We have engineered the ecdysone-inducible mammalian expression system for general retroviral delivery to cultured mammalian cells. We inducibly expressed PTEN in the glioblastoma cell line, U87MG, lacking this gene. Because nearly all cells are recruited on induction, we find both up- and down-regulated genes by cDNA microarray analysis. The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-beta gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer organism description > virus
CSHL Authors:	Hannon, Gregory J. Mittal, Vivek Wigler, Michael H. Chang, Kenneth
Communities:	CSHL labs > Hannon lab CSHL labs > Mittal lab CSHL labs > Wigler lab CSHL labs > Chang lab
Depositing User:	CSHL Librarian
Date:	November 2001
Date Deposited:	05 Apr 2012 18:31
Last Modified:	22 Nov 2019 19:03
PMCID:	PMC60821
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26005

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