Cellular Anatomy of the Mouse Primary Motor Cortex

Muñoz-Castañeda, Rodrigo, Zingg, Brian, Matho, Katherine, Wang, Quanxin, Chen, Xiaoyin, Foster, Nicholas, Narasimhan, Arun, Li, Anan, Hirokawa, Karla, Huo, Bingxing, Bannerjee, Samik, Korobkova, Laura, Park, Chris Sin, Park, Young-Gyun, Bienkowski, Michael, Chon, Uree, Wheeler, Diek, Li, Xiangning, Wang, Yun, Kelly, Kathleen, An, Xu, Attili, Sarojini, Bowman, Ian, Bludova, Anastasiia, Cetin, Ali, Ding, Liya, Drewes, Rhonda, D’Orazi, Florence, Elowsky, Corey, Fischer, Stephan, Galbavy, William, Gao, Lei, Gillis, Jesse, Groblewski, Peter, Gou, Lin, Hahn, Joel, Hatfield, Joshua, Hintiryan, Houri, Huang, Jason, Kondo, Hideki, Kuang, Xiuli, Lesnar, Philip, Li, Xu, Li, Yaoyao, Lin, Mengkuan, Liu, Lijuan, Lo, Darrick, Mizrachi, Judith, Mok, Stephanie, Naeemi, Maitham, Nicovich, Philip, Palaniswamy, Ramesh, Palmer, Jason, Qi, Xiaoli, Shen, Elise, Sun, Yu-Chi, Tao, Huizhong, Wakemen, Wayne, Wang, Yimin, Xie, Peng, Yao, Shenqin, Yuan, Jin, Zhu, Muye, Ng, Lydia, Zhang, Li, Lim, Byung Kook, Hawrylycz, Michael, Gong, Hui, Gee, James, Kim, Yongsoo, Peng, Hanchuan, Chuang, Kwanghun, Yang, William, Luo, Qingming, Mitra, Partha, Zador, Anthony, Zeng, Hongkui, Ascoli, Giorgio, Huang, Josh, Osten, Pavel, Harris, Julie, Dong, Hong-Wei (October 2020) Cellular Anatomy of the Mouse Primary Motor Cortex. BioRxiv. (Unpublished)

PDF 2020.MunozCastaneda.MousePrimaryMotorCortex.pdf Download (13MB)

Abstract

An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits and systems can be integrated and interpreted. Here, through a collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based description of one brain structure - the primary motor cortex upper limb area (MOp-ul) of the mouse. Applying state-of-the-art labeling, imaging, computational, and neuroinformatics tools, we delineated the MOp-ul within the Mouse Brain 3D Common Coordinate Framework (CCF). We defined over two dozen MOp-ul projection neuron (PN) types by their anterograde targets; the spatial distribution of their somata defines 11 cortical sublayers, a significant refinement of the classic notion of cortical laminar organization. We further combine multiple complementary tracing methods (classic tract tracing, cell type-based anterograde, retrograde, and transsynaptic viral tracing, high-throughput BARseq, and complete single cell reconstruction) to systematically chart cell type-based MOp input-output streams. As PNs link distant brain regions at synapses as well as host cellular gene expression, our construction of a PN type resolution MOp-ul wiring diagram will facilitate an integrated analysis of motor control circuitry across the molecular, cellular, and systems levels. This work further provides a roadmap towards a cellular resolution description of mammalian brain architecture.

Item Type:	Paper
Subjects:	Investigative techniques and equipment > brain atlas organs, tissues, organelles, cell types and functions > tissues types and functions > motor cortex organism description > animal > mammal > rodent > mouse
CSHL Authors:	Matho, Katherine Chen, Xiaoyin Narasimhan, Arun Fischer, Stephan Gillis, Jesse Palaniswamy, Ramesh Sun, Yu-Chi Mitra, Partha P. Zador, Anthony M. Osten, Pavel An, Xu Banerjee, Samik Bludova, Anastasiia Drewes, Rhonda Elowsky, Corey Galbavy, William Hatfield, Joshua Huang, Z. Josh Huo, Bing-Xing Kelly, Kathleen Kondo, Hideki Li, Xu Lin, Meng Kuan Mizrachi, Judith Munoz-Castaneda, Rodrigo Palmer, Jason Qi, Xiaoli
Communities:	CSHL labs > Gillis Lab CSHL labs > Huang lab CSHL labs > Mitra lab CSHL labs > Osten lab CSHL labs > Zador lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	2 October 2020
Date Deposited:	06 May 2021 20:36
Last Modified:	06 May 2021 20:36
Related URLs:	bioRxiv
URI:	https://repository.cshl.edu/id/eprint/39992

Actions (login required)

Administrator's edit/view item