Cellular Anatomy of the Mouse Primary Motor Cortex

Muñoz-Castañeda, Rodrigo, Zingg, Brian, Matho, Katherine, Wang, Quanxin, Chen, Xiaoyin, Foster, Nicholas, Narasimhan, Arun, Li, Anan, Hirokawa, Karla, Huo, Bingxing, Bannerjee, Samik, Korobkova, Laura, Park, Chris Sin, Park, Young-Gyun, Bienkowski, Michael, Chon, Uree, Wheeler, Diek, Li, Xiangning, Wang, Yun, Kelly, Kathleen, An, Xu, Attili, Sarojini, Bowman, Ian, Bludova, Anastasiia, Cetin, Ali, Ding, Liya, Drewes, Rhonda, D’Orazi, Florence, Elowsky, Corey, Fischer, Stephan, Galbavy, William, Gao, Lei, Gillis, Jesse, Groblewski, Peter, Gou, Lin, Hahn, Joel, Hatfield, Joshua, Hintiryan, Houri, Huang, Jason, Kondo, Hideki, Kuang, Xiuli, Lesnar, Philip, Li, Xu, Li, Yaoyao, Lin, Mengkuan, Liu, Lijuan, Lo, Darrick, Mizrachi, Judith, Mok, Stephanie, Naeemi, Maitham, Nicovich, Philip, Palaniswamy, Ramesh, Palmer, Jason, Qi, Xiaoli, Shen, Elise, Sun, Yu-Chi, Tao, Huizhong, Wakemen, Wayne, Wang, Yimin, Xie, Peng, Yao, Shenqin, Yuan, Jin, Zhu, Muye, Ng, Lydia, Zhang, Li, Lim, Byung Kook, Hawrylycz, Michael, Gong, Hui, Gee, James, Kim, Yongsoo, Peng, Hanchuan, Chuang, Kwanghun, Yang, William, Luo, Qingming, Mitra, Partha, Zador, Anthony, Zeng, Hongkui, Ascoli, Giorgio, Huang, Josh, Osten, Pavel, Harris, Julie, Dong, Hong-Wei (October 2020) Cellular Anatomy of the Mouse Primary Motor Cortex. BioRxiv. (Unpublished)

[thumbnail of 2020.MunozCastaneda.MousePrimaryMotorCortex.pdf] PDF
2020.MunozCastaneda.MousePrimaryMotorCortex.pdf
Download (13MB)

Abstract

An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits and systems can be integrated and interpreted. Here, through a collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based description of one brain structure - the primary motor cortex upper limb area (MOp-ul) of the mouse. Applying state-of-the-art labeling, imaging, computational, and neuroinformatics tools, we delineated the MOp-ul within the Mouse Brain 3D Common Coordinate Framework (CCF). We defined over two dozen MOp-ul projection neuron (PN) types by their anterograde targets; the spatial distribution of their somata defines 11 cortical sublayers, a significant refinement of the classic notion of cortical laminar organization. We further combine multiple complementary tracing methods (classic tract tracing, cell type-based anterograde, retrograde, and transsynaptic viral tracing, high-throughput BARseq, and complete single cell reconstruction) to systematically chart cell type-based MOp input-output streams. As PNs link distant brain regions at synapses as well as host cellular gene expression, our construction of a PN type resolution MOp-ul wiring diagram will facilitate an integrated analysis of motor control circuitry across the molecular, cellular, and systems levels. This work further provides a roadmap towards a cellular resolution description of mammalian brain architecture.

Item Type: Paper
Subjects: Investigative techniques and equipment > brain atlas
organs, tissues, organelles, cell types and functions > tissues types and functions > motor cortex
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Gillis Lab
CSHL labs > Huang lab
CSHL labs > Mitra lab
CSHL labs > Osten lab
CSHL labs > Zador lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 2 October 2020
Date Deposited: 06 May 2021 20:36
Last Modified: 06 May 2021 20:36
Related URLs:
URI: https://repository.cshl.edu/id/eprint/39992

Actions (login required)

Administrator's edit/view item Administrator's edit/view item