Martienssen, R. A., Rabinowicz, P. D., O'Shaughnessy, A., McCombie, W. R. (2004) Sequencing the maize genome. Current Opinion in Plant Biology, 7 (2). pp. 102-107. ISSN 13695266 (ISSN)
Abstract
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C0t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | BAC Bacterial artificial chromosome C0 DNA concentration at time zero EST Expressed sequence tag Methylation filtration MF Re-association time t chromosome map genetics genome maize methodology review Chromosomes, Artificial, Bacterial Genome, Plant Physical Chromosome Mapping Zea mays Bacteria (microorganisms) Embryophyta |
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > plant > maize bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | 2004 |
| Date Deposited: | 23 Apr 2013 15:18 |
| Last Modified: | 23 Apr 2013 15:18 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28246 |
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