Palmer, L. E., Rabinowicz, P. D., O'Shaughnessy, A. L., Balija, V. S., Nascimento, L. U., Dike, S., de la Bastide, M., Martienssen, R. A., McCombie, W. R. (December 2003) Maize genome sequencing by methylation filtrations. Science, 302 (5653). pp. 2115-2117. ISSN 0036-8075
Abstract
Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA methylation transposable elements draft sequence gene retrotransposons grasses regions plants |
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment organism description > plant > maize bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | 19 December 2003 |
| Date Deposited: | 27 Mar 2013 16:05 |
| Last Modified: | 27 Mar 2013 16:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28021 |
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