Genes and transposons are differentially methylated in plants, but not in mammals

Rabinowicz, P. D., Palmer, L. E., May, B. P., Hemann, M. T., Lowe, S. W., McCombie, W. R., Martienssen, R. A. (December 2003) Genes and transposons are differentially methylated in plants, but not in mammals. Genome Research, 13 (12). pp. 2658-64. ISSN 1088-9051

URL: http://www.ncbi.nlm.nih.gov/pubmed/14656970

DOI: 10.1101/gr.1784803

Abstract

DNA methylation is found in many eukaryotes, but its function is still controversial. We have studied the methylation of plant and animal genomes using a PCR-based technique amenable for high throughput. Repetitive elements are methylated in both organisms, but whereas most mammalian exons are methylated, plant exons are not. Thus, targeting of methylation specifically to transposons appears to be restricted to plants. We propose that the mechanistic basis of this difference may involve RNA interference. Sequencing strategies that depend on differential methylation are predicted to have different outcomes in plant and mammalian genomes.

Item Type:	Paper
Uncontrolled Keywords:	Animals DNA Methylation DNA Transposable Elements Fibroblasts chemistry metabolism Genes Genes Plant Genome Genome Human Genome Plant Humans Lung cytology embryology Mice Mice Inbred C57BL Mice Transgenic Spleen chemistry embryology metabolism Zea mays/genetics
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:	Hemann, Michael T. Lowe, Scott W. Martienssen, Robert A. May, Bruce McCombie, W. Richard Palmer, Lance E. Rabinowicz, Pablo D.
Communities:	CSHL labs > Lowe lab CSHL labs > Martienssen lab CSHL labs > McCombie lab
Depositing User:	Matt Covey
Date:	December 2003
Date Deposited:	01 Apr 2013 18:56
Last Modified:	01 Apr 2013 18:56
PMCID:	PMC403807
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28012

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