Xu, H. P., Rajavashisth, T., Grewal, N., Jung, V., Riggs, M., Rodgers, L., Wigler, M. H. (July 1992) A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe. Mol Biol Cell, 3 (7). pp. 721-34. ISSN 1059-1524 (Print)
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Abstract
Byr3 was selected as a multicopy suppressor of the sporulation defects of diploid Schizosaccharomyces pombe cells that lack ras1. Like cells mutant at byr1 and byr2, two genes that encode putative protein kinases and that in multiple copies are also suppressors of the sporulation defects of ras1 null diploid cells, cells mutant at byr3 are viable but defective in conjugation. Nucleic acid sequence indicates byr3 has the capacity to encode a protein with seven zinc finger binding domains, similar in structure to the cellular nucleic acid binding protein (CNBP), a human protein that was identified on the basis of its ability to bind DNA. Expression of CNBP in yeast can partially suppress conjugation defects of cells lacking byr3.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cloning Molecular Conjugation, Genetic DNA, Fungal genetics DNA-Binding Proteins genetics Fungal Proteins genetics Genes Fungal Genes, Suppressor Genes ras Humans In Vitro Molecular Sequence Data Oligodeoxyribonucleotides chemistry Precipitin Tests Recombinant Fusion Proteins genetics Restriction Mapping Schizosaccharomyces genetics Schizosaccharomyces pombe Proteins Sex Differentiation Spores Fungal Zinc Fingers |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > suppressor organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab CSHL labs > Stillman lab |
| Depositing User: | CSHL Librarian |
| Date: | July 1992 |
| Date Deposited: | 06 Apr 2012 18:52 |
| Last Modified: | 17 Nov 2016 16:41 |
| PMCID: | PMC275630 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25950 |
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