Xu, H. P., White, M., Marcus, S., Wigler, M. H. (January 1994) Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe. Mol Cell Biol, 14 (1). pp. 50-8. ISSN 0270-7306 (Print)
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Abstract
We have shown that the expression of mam2, the gene encoding the Schizosaccharomyces pombe P-factor pheromone receptor, is dependent upon components of the pheromone signal transduction pathway, including Ras1, Gpa1, Byr1 and Byr2, each of which is required for both conjugation and sporulation. Studies of the expression of mam2 in mutant S. pombe cells confirm previous conclusions, based on the ability of cells to sporulate, that the Byr1 protein kinase acts downstream of the Byr2 protein kinase and that both act downstream of Ras1, the S. pombe RAS homolog, and Gpa1, the G alpha component that mediates the occupancy of the mam2 receptor. In addition, our present studies show that Ras1 and Gpa1 each act downstream from the other and hence act in concert. The Spk1 kinase, which is required for conjugation and sporulation and which is a structural and functional homolog of the vertebrate MAP kinases, is not required for mam2 expression.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Conjugation Genetic DNA Primers genetics DNA, Fungal genetics Fungal Proteins metabolism GTP-Binding Protein alpha Subunits GTP-Binding Proteins metabolism Gene Expression Regulation, Fungal Genes, Fungal Heterotrimeric GTP-Binding Proteins Molecular Sequence Data Protein Kinases metabolism Receptors, Mating Factor Receptors, Peptide genetics metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces genetics metabolism Schizosaccharomyces pombe Proteins Signal Transduction Spores Fungal genetics metabolism Transcription Factors ras Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | January 1994 |
| Date Deposited: | 06 Apr 2012 18:36 |
| Last Modified: | 04 Nov 2016 20:02 |
| PMCID: | PMC358355 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25947 |
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