BPTF Maintains Chromatin Accessibility and the Self-Renewal Capacity of Mammary Gland Stem Cells

Frey, W. D., Chaudhry, A., Slepicka, P. F., Ouellette, A. M., Kirberger, S. E., Pomerantz, W. C. K., Hannon, G. J., Dos Santos, C. O. (July 2017) BPTF Maintains Chromatin Accessibility and the Self-Renewal Capacity of Mammary Gland Stem Cells. Stem Cell Reports, 9 (1). pp. 23-31. ISSN 2213-6711 (Public Dataset)

Abstract

Chromatin remodeling is a key requirement for transcriptional control of cellular differentiation. However, the factors that alter chromatin architecture in mammary stem cells (MaSCs) are poorly understood. Here, we show that BPTF, the largest subunit of the NURF chromatin remodeling complex, is essential for MaSC self-renewal and differentiation of mammary epithelial cells (MECs). BPTF depletion arrests cells at a previously undefined stage of epithelial differentiation that is associated with an incapacity to achieve the luminal cell fate. Moreover, genome-wide analysis of DNA accessibility following genetic or chemical inhibition, suggests a role for BPTF in maintaining the open chromatin landscape at enhancers regions in MECs. Collectively, our study implicates BPTF in maintaining the unique epigenetic state of MaSCs.

Item Type:	Paper
Uncontrolled Keywords:	Bptf chromatin remodeling enhancer landscape gene regulation mammary gland development mammary stem cells
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromatin remodeling bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation organs, tissues, organelles, cell types and functions > tissues types and functions > mammary gland organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Frey, Wesley Chaudhary, Fauzia Hannon, Gregory J. Dos Santos, Camila O.
Communities:	CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL labs > Dos Santos lab CSHL labs > Hannon lab CSHL Cancer Center Program > Cancer Genetics and Genomics Program CSHL Cancer Center Program > Cellular Communication in Cancer Program
Depositing User:	Matt Covey
Date:	11 July 2017
Date Deposited:	21 Jun 2017 20:06
Last Modified:	26 Oct 2020 16:54
PMCID:	PMC5783326
Related URLs:	Publisher
Dataset ID:	GEO: GSE98004
URI:	https://repository.cshl.edu/id/eprint/35014

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