Frey, W. D., Chaudhry, A., Slepicka, P. F., Ouellette, A. M., Kirberger, S. E., Pomerantz, W. C. K., Hannon, G. J., Dos Santos, C. O.
(July 2017)
BPTF Maintains Chromatin Accessibility and the Self-Renewal Capacity of Mammary Gland Stem Cells.
Stem Cell Reports, 9 (1).
pp. 23-31.
ISSN 2213-6711
(Public Dataset)
Abstract
Chromatin remodeling is a key requirement for transcriptional control of cellular differentiation. However, the factors that alter chromatin architecture in mammary stem cells (MaSCs) are poorly understood. Here, we show that BPTF, the largest subunit of the NURF chromatin remodeling complex, is essential for MaSC self-renewal and differentiation of mammary epithelial cells (MECs). BPTF depletion arrests cells at a previously undefined stage of epithelial differentiation that is associated with an incapacity to achieve the luminal cell fate. Moreover, genome-wide analysis of DNA accessibility following genetic or chemical inhibition, suggests a role for BPTF in maintaining the open chromatin landscape at enhancers regions in MECs. Collectively, our study implicates BPTF in maintaining the unique epigenetic state of MaSCs.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Bptf
chromatin remodeling
enhancer landscape
gene regulation
mammary gland development
mammary stem cells |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromatin remodeling
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
organs, tissues, organelles, cell types and functions > tissues types and functions > mammary gland
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells |
| CSHL Authors: |
|
| Communities: |
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL labs > Dos Santos lab
CSHL labs > Hannon lab
CSHL Cancer Center Program > Cancer Genetics and Genomics Program
CSHL Cancer Center Program > Cellular Communication in Cancer Program |
| Depositing User: |
Matt Covey
|
| Date: |
11 July 2017 |
| Date Deposited: |
21 Jun 2017 20:06 |
| Last Modified: |
26 Oct 2020 16:54 |
| PMCID: |
PMC5783326 |
| Related URLs: |
|
| Dataset ID: |
|
| URI: |
https://repository.cshl.edu/id/eprint/35014 |
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