Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase

Colicelli, J., Birchmeier, C., Michaeli, T., O'Neill, K., Riggs, M., Wigler, M. H. (May 1989) Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase. Proc Natl Acad Sci U S A, 86 (10). pp. 3599-603. ISSN 0027-8424

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Abstract

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.

Item Type:	Paper
Additional Information:
Uncontrolled Keywords:	3',5'-Cyclic-Nucleotide Phosphodiesterase genetics Amino Acid Sequence Animals Base Sequence Cloning Molecular DNA genetics Gene Expression Regulation Genetic Complementation Test Heat Molecular Sequence Data Rats Restriction Mapping Saccharomyces cerevisiae genetics
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
CSHL Authors:	Wigler, Michael H. Colicelli, John Birchmeier, Carmen Michaeli, Tamar O'Neill, Kathy Riggs, Michael
Communities:	CSHL labs > Wigler lab
Depositing User:	CSHL Librarian
Date:	May 1989
Date Deposited:	19 Apr 2012 14:21
Last Modified:	04 May 2022 20:04
PMCID:	PMC287185
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26171

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