Colicelli, J., Birchmeier, C., Michaeli, T., O'Neill, K., Riggs, M., Wigler, M. H.
(May 1989)
Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
Proc Natl Acad Sci U S A, 86 (10).
pp. 3599-603.
ISSN 0027-8424
Abstract
A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.
| Item Type: |
Paper
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| Additional Information: |
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| Uncontrolled Keywords: |
3',5'-Cyclic-Nucleotide Phosphodiesterase genetics
Amino Acid Sequence
Animals
Base Sequence
Cloning Molecular
DNA genetics
Gene Expression Regulation
Genetic Complementation Test
Heat
Molecular Sequence Data
Rats
Restriction Mapping
Saccharomyces cerevisiae genetics |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization |
| CSHL Authors: |
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| Communities: |
CSHL labs > Wigler lab |
| Depositing User: |
CSHL Librarian
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| Date: |
May 1989 |
| Date Deposited: |
19 Apr 2012 14:21 |
| Last Modified: |
04 May 2022 20:04 |
| PMCID: |
PMC287185 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26171 |
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