Insertional mutagenesis of the maize P gene by intragenic transposition of Ac

Athma, P., Grotewold, E., Peterson, T. (1992) Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics, 131 (1). pp. 199-209. ISSN 0016-6731 (Print)0016-6731 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1317315

Abstract

The P-rr allele of the maize P gene regulates the synthesis of pigments derived from flavan-4-ol in the pericarp, cob glumes and other floral organs. We characterized 21 P alleles derived by intragenic transposition of Ac from three known positions. Ac transpositions can occur in either direction in the P gene, and with no apparent minimum distance: in one case Ac transposed just 6 bp from its original insertion site. However, the distribution of transposed Ac elements was markedly nonrandom: of 19 transposed Ac elements derived from a single Ac donor, 15 were inserted in a 1.1-kb region at the 5' end of P, while none had inserted in an adjacent 3.2-kb intronic region. All of the Ac insertions affect both pericarp and cob glume pigmentation, providing further evidence that the P-rr allele contains a single gene required for both pericarp and cob glume pigmentation. The distribution of the inserted Ac elements and the phenotype conditioned by each allele suggests a structure of P-rr which is similar to that previously determined molecularly. Possible explanations for the nonrandom distribution of transposed Ac elements are discussed.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Cloning, Molecular *DNA Transposable Elements Gene Expression Molecular Sequence Data *Mutagenesis, Site-Directed Restriction Mapping Substrate Specificity Zea mays/*genetics
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs > Grotewold lab
Depositing User: Matt Covey
Date Deposited: 29 Sep 2015 16:50
Last Modified: 29 Sep 2015 16:50
PMCID: PMC1204954
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31798

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