Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator

Martienssen, R. A., Barkan, A., Freeling, M., Taylor, W. C. (1989) Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator. EMBO J, 8 (6). pp. 1633-1639. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/2548850
DOI: PMC401002

Abstract

The maize photosynthetic mutant hcf106 has a distinctive and unusual thylakoid membrane organization, and fails to accumulate three of the five thylakoid membrane protein complexes. This mutant arose in a Robertson's Mutator background, and shows somatic instability typical of a transposon-induced allele. In addition, hcf106 is suppressed when Mu1 elements are inactive and modified in their terminal inverted repeats. Thus plants homozygous for the mutant allele adopt a mutant phenotype only when Mu1 elements are active and unmodified. DNA from the mutant allele has been cloned by 'transposon-tagging' using the transposon Mu1, and the identity of the clone confirmed by observing somatic excision of the transposon in a revertant sector. A 1.2 kb transcript homologous to the cloned DNA is found in wild-type and suppressed seedlings, but is not found in mutant seedlings, suggesting that suppression is mediated at the level of transcript accumulation.

Item Type: Paper
Uncontrolled Keywords: Alleles Chloroplasts Cloning, Molecular DNA Transposable Elements Genes Membrane Proteins Mutation Phenotype Photosynthesis Suppression, Genetic Transcription, Genetic Zea mays
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > plant > maize
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 1989
Date Deposited: 07 Jan 2014 15:22
Last Modified: 07 Jan 2014 15:22
URI: http://repository.cshl.edu/id/eprint/29194

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