Martienssen, R. A., Barkan, A., Freeling, M., Taylor, W. C. (1989) Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator. EMBO J, 8 (6). pp. 1633-1639. ISSN 0261-4189
Abstract
The maize photosynthetic mutant hcf106 has a distinctive and unusual thylakoid membrane organization, and fails to accumulate three of the five thylakoid membrane protein complexes. This mutant arose in a Robertson's Mutator background, and shows somatic instability typical of a transposon-induced allele. In addition, hcf106 is suppressed when Mu1 elements are inactive and modified in their terminal inverted repeats. Thus plants homozygous for the mutant allele adopt a mutant phenotype only when Mu1 elements are active and unmodified. DNA from the mutant allele has been cloned by 'transposon-tagging' using the transposon Mu1, and the identity of the clone confirmed by observing somatic excision of the transposon in a revertant sector. A 1.2 kb transcript homologous to the cloned DNA is found in wild-type and suppressed seedlings, but is not found in mutant seedlings, suggesting that suppression is mediated at the level of transcript accumulation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Alleles Chloroplasts Cloning, Molecular DNA Transposable Elements Genes Membrane Proteins Mutation Phenotype Photosynthesis Suppression, Genetic Transcription, Genetic Zea mays |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > plant > maize Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab |
| Depositing User: | Matt Covey |
| Date: | 1989 |
| Date Deposited: | 07 Jan 2014 15:22 |
| Last Modified: | 07 Jan 2014 15:22 |
| URI: | https://repository.cshl.edu/id/eprint/29194 |
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