The structure of human argonaute-2 in complex with miR-20a

Elkayam, E., Kuhn, C. D., Tocilj, A., Haase, A. D., Greene, E. M., Hannon, G. J., Joshua-Tor, L. (2012) The structure of human argonaute-2 in complex with miR-20a. Cell, 150 (1). pp. 100-110. ISSN 00928674 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/22682761
DOI: 10.1016/j.cell.2012.05.017

Abstract

Argonaute proteins lie at the heart of the RNA-induced silencing complex (RISC), wherein they use small RNA guides to recognize targets. Initial insight into the architecture of Argonautes came from studies of prokaryotic proteins, revealing a crescent-shaped base made up of the amino-terminal, PAZ, middle, and PIWI domains. The recently reported crystal structure of human Argonaute-2 (hAgo2), the "slicer" in RNA interference, in complex with a mixed population of RNAs derived from insect cells provides insight into the architecture of a eukaryotic Argonaute protein with defined biochemical and biological functions. Here, we report the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 Å resolution. The miRNA is anchored at both ends by the Mid and PAZ domains and makes several kinks and turns along the binding groove. Interestingly, miRNA binding confers remarkable stability on hAgo2, locking this otherwise flexible enzyme into a stable conformation. © 2012 Elsevier Inc.

Item Type: Paper
Uncontrolled Keywords: argonaute 2 protein microRNA 20a recombinant protein animal cell article complex formation controlled study crystal structure female nonhuman nucleotide sequence priority journal protein conformation protein domain protein function protein RNA binding protein stability protein structure RNA sequence theoretical model Eukaryota Hexapoda Prokaryota
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Mass Spectrometry Service
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL Post Doctoral Fellows
CSHL labs > Hannon lab
CSHL labs > Joshua-Tor lab
Depositing User: Matt Covey
Date Deposited: 09 Jan 2013 16:06
Last Modified: 05 Sep 2017 16:01
PMCID: PMC3464090
Related URLs:
Dataset ID:
URI: http://repository.cshl.edu/id/eprint/26418

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