Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe

Marcus, S., Polverino, A., Chang, E., Robbins, D., Cobb, M. H., Wigler, M. H. (June 1995) Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc Natl Acad Sci U S A, 92 (13). pp. 6180-4. ISSN 0027-8424 (Print)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/7597098
DOI: 10.1073/pnas.92.13.6180

Abstract

We describe a protein kinase, Shk1, from the fission yeast Schizosaccharomyces pombe, which is structurally related to the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases. We provide genetic evidence for physical and functional interaction between Shk1 and the Cdc42 GTP-binding protein required for normal cell morphology and mating in S. pombe. We further show that expression of the STE20 gene complements the shk1 null mutation and that Shk1 is capable of signaling to the pheromone-responsive mitogen-activated protein kinase cascade in S. cerevisiae. Our results lead us to propose that signaling modules composed of small GTP-binding proteins and protein kinases related to Shk1, Ste20, and p65PAK, are highly conserved in evolution and participate in both cytoskeletal functions and mitogen-activated protein kinase signaling pathways.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins metabolism Comparative Study Crosses, Genetic DNA Primers Fungal Proteins metabolism GTP-Binding Proteins metabolism Gene Deletion Genes Fungal Mammals Molecular Sequence Data Protein Serine Threonine Kinases biosynthesis genetics metabolism Regulatory Sequences Nucleic Acid Saccharomyces cerevisiae enzymology Saccharomyces cerevisiae Proteins Schizosaccharomyces enzymology genetics physiology Schizosaccharomyces pombe Proteins Sequence Deletion Sequence Homology Amino Acid Signal Transduction beta-Galactosidase analysis biosynthesis cdc42 GTP-Binding Protein ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 20 June 1995
Date Deposited: 11 Apr 2012 21:01
Last Modified: 09 Nov 2017 19:29
PMCID: PMC41666
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26224

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