Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase

Colicelli, J., Birchmeier, C., Michaeli, T., O'Neill, K., Riggs, M., Wigler, M. H. (May 1989) Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase. Proc Natl Acad Sci U S A, 86 (10). pp. 3599-603. ISSN 0027-8424

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URL: https://www.ncbi.nlm.nih.gov/pubmed/2542941
DOI: 10.1073/pnas.86.10.3599

Abstract

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: 3',5'-Cyclic-Nucleotide Phosphodiesterase genetics Amino Acid Sequence Animals Base Sequence Cloning Molecular DNA genetics Gene Expression Regulation Genetic Complementation Test Heat Molecular Sequence Data Rats Restriction Mapping Saccharomyces cerevisiae genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
CSHL Authors:
Communities: CSHL labs > Wigler lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: May 1989
Date Deposited: 19 Apr 2012 14:21
Last Modified: 07 Jan 2019 19:49
PMCID: PMC287185
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26171

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