Isolation of the chicken thymidine kinase gene by plasmid rescue

Perucho, M., Hanahan, D., Lipsich, L., Wigler, M. H. (May 1980) Isolation of the chicken thymidine kinase gene by plasmid rescue. Nature, 285 (5762). pp. 207-210.

URL: https://www.ncbi.nlm.nih.gov/pubmed/6246445
DOI: 10.1038/285207a0

Abstract

The bacterial plasmid pBR322 was used as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. The chicken thymidine kinase (tk) gene was obtained as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > virus
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 22 May 1980
Date Deposited: 05 Apr 2012 15:45
Last Modified: 07 Nov 2016 20:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26027

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