Perucho, M., Hanahan, D., Lipsich, L., Wigler, M. H. (May 1980) Isolation of the chicken thymidine kinase gene by plasmid rescue. Nature, 285 (5762). pp. 207-210.
DOI: 10.1038/285207a0
Abstract
The bacterial plasmid pBR322 was used as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. The chicken thymidine kinase (tk) gene was obtained as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 22 May 1980 |
| Date Deposited: | 05 Apr 2012 15:45 |
| Last Modified: | 07 Nov 2016 20:45 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26027 |
Actions (login required)
 |
Administrator's edit/view item |