Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase

Toda, T., Cameron, S., Sass, P., Zoller, M., Wigler, M. H. (1987) Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell, 50 (2). pp. 277-288.

URL: https://www.ncbi.nlm.nih.gov/pubmed/3036373
DOI: 10.1016/0092-8674(87)90223-6

Abstract

We have isolated three genes (TPK1, TPK2, and TPK3) from the yeast Saccharomyces cerevisiae that encode the catalytic subunits of the cAMP-dependent protein kinase. Gene disruption experiments demonstrated that no two of the three genes are essential by themselves but at least one TPK gene is required for a cell to grow normally. Comparison of the predicted amino acid sequences of the TPK genes indicates conserved and variable domains. The carboxy-terminal 320 amino acid residues have more than 75% homology to each other and more than 50% homology to the bovine catalytic subunit. The amino-terminal regions show no homology to each other and are heterogeneous in length. The TPK1 gene carried on a multicopy plasmid can suppress both a temperature-sensitive ras2 gene and adenylate cyclase gene.

Item Type: Paper
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1987
Date Deposited: 05 Apr 2012 18:58
Last Modified: 17 Nov 2016 17:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25995

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