Toda, T., Cameron, S., Sass, P., Zoller, M., Wigler, M. H. (1987) Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell, 50 (2). pp. 277-288.
Abstract
We have isolated three genes (TPK1, TPK2, and TPK3) from the yeast Saccharomyces cerevisiae that encode the catalytic subunits of the cAMP-dependent protein kinase. Gene disruption experiments demonstrated that no two of the three genes are essential by themselves but at least one TPK gene is required for a cell to grow normally. Comparison of the predicted amino acid sequences of the TPK genes indicates conserved and variable domains. The carboxy-terminal 320 amino acid residues have more than 75% homology to each other and more than 50% homology to the bovine catalytic subunit. The amino-terminal regions show no homology to each other and are heterogeneous in length. The TPK1 gene carried on a multicopy plasmid can suppress both a temperature-sensitive ras2 gene and adenylate cyclase gene.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1987 |
| Date Deposited: | 05 Apr 2012 18:58 |
| Last Modified: | 17 Nov 2016 17:01 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25995 |
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