Homer, Joshua A, Sun, Shoujun, Koelln, Rebecca A, Moses, John E (January 2024) Protocol for producing phosphoramidate using phosphorus fluoride exchange click chemistry. STAR Protocols, 5 (1). p. 102824. ISSN 2666-1667
Preview |
PDF
2024_Homer_Protocol_for_producing_phosphoramidate.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (4MB) | Preview |
Abstract
Phosphorus fluoride exchange (PFEx) is a catalytic click reaction that involves exchanging high oxidation state P-F bonds with alcohol and amine nucleophiles, reliably yielding P-O- and P-N-linked compounds. Here, we describe steps for preparing a phosphoramidic difluoride and performing two sequential PFEx reactions to yield a phosphoramidate through careful catalyst selection. We then detail procedures for handling and quenching potentially toxic P-F-containing compounds to ensure user safety when conducting PFEx reactions. For complete details on the use and execution of this protocol, please refer to Sun et al.1.
| Item Type: | Paper |
|---|---|
| Subjects: | chemistry > techniques > click chemistry > Accelerated SuFEx Click Chemistry chemistry chemistry > techniques > click chemistry chemistry > techniques |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program CSHL Cancer Center Shared Resources > Mass Spectrometry Service CSHL labs > Moses lab CSHL Cancer Center Program > Cellular Communication in Cancer Program |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 12 January 2024 |
| Date Deposited: | 18 Jan 2024 18:10 |
| Last Modified: | 29 Apr 2024 18:23 |
| PMCID: | PMC10825769 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41403 |
Actions (login required)
 |
Administrator's edit/view item |