Variant phasing and haplotypic expression from long-read sequencing in maize

Wang, B., Tseng, E., Baybayan, P., Eng, K., Regulski, M., Jiao, Y., Wang, L., Olson, A., Chougule, K., Buren, P. V., Ware, D. (February 2020) Variant phasing and haplotypic expression from long-read sequencing in maize. Commun Biol, 3 (1). p. 78. ISSN 2399-3642 (Public Dataset)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/32071408

DOI: 10.1038/s42003-020-0805-8

Abstract

Haplotype phasing maize genetic variants is important for genome interpretation, population genetic analysis and functional analysis of allelic activity. We performed an isoform-level phasing study using two maize inbred lines and their reciprocal crosses, based on single-molecule, full-length cDNA sequencing. To phase and analyze transcripts between hybrids and parents, we developed IsoPhase. Using this tool, we validated the majority of SNPs called against matching short-read data from embryo, endosperm and root tissues, and identified allele-specific, gene-level and isoform-level differential expression between the inbred parental lines and hybrid offspring. After phasing 6907 genes in the reciprocal hybrids, we annotated the SNPs and identified large-effect genes. In addition, we identified parent-of-origin isoforms, distinct novel isoforms in maize parent and hybrid lines, and imprinted genes from different tissues. Finally, we characterized variation in cis- and trans-regulatory effects. Our study provides measures of haplotypic expression that could increase accuracy in studies of allelic expression.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment organism description > plant > maize bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > alleles Investigative techniques and equipment > assays bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > single nucleotide polymorphism > haplotype Investigative techniques and equipment > assays > long-read sequencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > single nucleotide polymorphism
CSHL Authors:	Ware, Doreen H. Wang, Bo Regulski, Michael R. Jiao, Yinping Wang, Liya Olson, Andrew J. Chougule, Kapeel Van Buren, Peter
Communities:	CSHL labs > Ware lab
Depositing User:	Adrian Gomez
Date:	18 February 2020
Date Deposited:	21 Feb 2020 15:40
Last Modified:	01 Feb 2024 19:14
PMCID:	PMC7028979
Related URLs:	Publisher
Dataset ID:	The IsoPhase tool developed in this study is available in the GitHub repository: https://github.com/magdoll/cdna_cupcake. The related files generated from the code are stored at Zenodo repository (https://doi.org/10.5281/zenodo.2611319) The data generated in this study, including PacBio Iso-Seq reads and Illumina short reads, have been submitted to ArrayExpress (https://www.ebi.ac.uk/arrayexpress/) under accession numbers E-MTAB-7837 and E-MTAB-7394.
URI:	https://repository.cshl.edu/id/eprint/39069

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