p21-Containing cyclin kinases exist in both active and inactive states

Zhang, H., Hannon, G. J., Beach, D. (1994) p21-Containing cyclin kinases exist in both active and inactive states. Genes and Development, 8 (15). pp. 1750-1758. ISSN 08909369 (ISSN)

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Abstract

In normal fibroblasts CDKs exist predominantly in p21/PCNA/cyclin/CDK quaternary complexes, whereas in p53-deficient cells, p21 expression is depressed and the kinases are reduced to a cyclin/CDK binary state. p21 is a universal cyclin kinase inhibitor, but we show here that p21-containing complexes exist in both catalytically active and inactive forms. This finding challenges the current view that active cyclin kinases function only in the binary state and reveals the subtlety with which tumor-suppressor proteins modulate the cell cycle.

Item Type:	Paper
Uncontrolled Keywords:	cell cycle progression human fibroblasts kinase inhibitor p21-containing cyclin kinase quaternary complex cyclin b cycline histone h1 hybrid protein myelin basic protein protamine kinase protein kinase protein p21 animal cell article cell cycle complex formation enzyme activation fibroblast human human cell insect nonhuman priority journal tumor cell line Base Sequence Catalysis Cell Line, Transformed Cells, Cultured Centrifugation, Density Gradient Chromatography, Gel Cloning, Molecular Cyclin-Dependent Kinases Hela Cells Models, Chemical Molecular Sequence Data Precipitin Tests Proliferating Cell Nuclear Antigen Proto-Oncogene Protein p21(ras) Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured Animalia Insecta
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p21 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Beach, David H. Hannon, Gregory J.
Communities:	CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	1994
Date Deposited:	09 Jan 2013 16:58
Last Modified:	14 Jan 2013 22:08
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26487

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