Marcus, S., Polverino, A., Barr, M., Wigler, M. H. (August 1994) Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci U S A, 91 (16). pp. 7762-6. ISSN 0027-8424 (Print)
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Abstract
We present genetic evidence for complex formation of STE5 and the STE11, STE7, and FUS3 protein kinases, the pheromone-responsive mitogen-activated protein kinase module of Saccharomyces cerevisiae. Interaction between STE5 and STE11 is not dependent on STE7, and interaction between STE5 and STE7 does not require STE11. The N-terminal regulatory domain of STE11 is both necessary and sufficient for interaction with STE5. Interaction between STE7 and STE11 is bridged by STE5, suggesting the formation of a multiprotein complex. We also demonstrate biochemical interaction between STE5 and STE11 by using a combination of bacterially expressed fusion proteins and extracts prepared from yeast. Our results suggest that STE5 is a scaffolding protein that facilitates interactions between components of the pheromone-responsive mitogen-activated protein kinase module. We further propose that such scaffolding proteins serve to inhibit cross-talk between functionally unrelated mitogen-activated protein kinase modules within the same cell.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adaptor Proteins Signal Transducing Ca(2+)-Calmodulin Dependent Protein Kinase genetics metabolism Carrier Proteins Fungal Proteins genetics metabolism MAP Kinase Kinase 1 MAP Kinase Kinase Kinases metabolism Mitogen Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Pheromones metabolism Protein Kinases genetics metabolism Protein Serine Threonine Kinases metabolism Protein Tyrosine Kinase metabolism Saccharomyces cerevisiae physiology Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Signal Transduction Transcription Factors |
| Subjects: | organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 2 August 1994 |
| Date Deposited: | 12 Apr 2012 14:48 |
| Last Modified: | 09 Nov 2017 19:37 |
| PMCID: | PMC44482 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26223 |
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