Field, J., Xu, H. P., Michaeli, T., Ballester, R., Sass, P., Wigler, M. H., Colicelli, J. (January 1990) Mutations of the adenylyl cyclase gene that block RAS function in Saccharomyces cerevisiae. Science, 247 (4941). pp. 464-7. ISSN 0036-8075
Abstract
The interaction between RAS proteins and adenylyl cyclase was studied by using dominant interfering mutations of adenylyl cyclase from the yeast Saccharomyces cerevisiae. RAS proteins activate adenylyl cyclase in this organism. A plasmid expressing a catalytically inactive adenylyl cyclase was found to interfere dominantly with this activation. The interfering region mapped to the leucine-rich repeat region of adenylyl cyclase, which is homologous to domains present in several other proteins and is thought to participate in protein-protein interactions.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Adenylate Cyclase genetics metabolism Codon Deoxyribonucleases Type II Site-Specific Escherichia coli genetics Fungal Proteins metabolism Genes, Fungal Leucine Mutation Plasmids Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae enzymology genetics Sequence Homology Nucleic Acid Transformation Genetic ras Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 26 January 1990 |
| Date Deposited: | 13 Apr 2012 20:15 |
| Last Modified: | 18 Nov 2016 17:19 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26183 |
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