Ballester, R., Michaeli, T., Ferguson, K., Xu, H. P., McCormick, F., Wigler, M. H. (November 1989) Genetic analysis of mammalian GAP expressed in yeast. Cell, 59 (4). pp. 681-6. ISSN 0092-8674
Abstract
We have designed a vector to express the mammalian GAP protein in the yeast S. cerevisiae. When expressed in yeast, GAP inhibits the function of the human H-rasgly12 protein, but not that of the H-rasval12 protein, and complements the loss of IRA1. IRA1 is a yeast gene that encodes a protein with homology to GAP and acts upstream of RAS. Mammalian GAP can therefore function in yeast and interact with yeast RAS. Because expression of GAP complements ira1-mutants, we propose that GAP shares some biochemical functions with IRA1. Other studies indicate that IRA1 controls the level of RAS activity, presumably by regulating GTP hydrolysis. By analogy, we propose that GAP may play a similar role.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Cloning, Molecular methods Escherichia coli genetics GTPase Activating Proteins Gene Expression Genes Structural Genes ras Genetic Vectors Heat Plasmids Protein Biosynthesis Proteins genetics Restriction Mapping Saccharomyces cerevisiae genetics ras GTPase-Activating Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 17 November 1989 |
| Date Deposited: | 19 Apr 2012 16:26 |
| Last Modified: | 18 Nov 2016 17:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26152 |
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