Ballester, R., Marchuk, D., Boguski, M., Saulino, A., Letcher, R., Wigler, M. H., Collins, F.
(November 1990)
The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins.
Cell, 63 (4).
pp. 851-9.
ISSN 0092-8674
Abstract
The von Recklinghausen neurofibromatosis locus, NF1, encodes a protein with homology restricted to the catalytic region of the RAS GTPase-activating protein, GAP, and with extensive homology to the IRA1 and IRA2 gene products of the yeast S. cerevisiae. A segment of the NF1 cDNA gene, expressed in yeast, can complement loss of IRA function and can inhibit both wild-type and mutant activated human H-ras genes that are coexpressed in yeast. Yeast expressing the NF1 segment have increased H-ras GTPase-stimulating activity. These studies indicate that the NF1 gene product can interact with RAS proteins and demonstrate structural and functional similarities and differences among the GAP, IRA1, IRA2, and NF1 proteins.
| Item Type: |
Paper
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| Additional Information: |
|
| Uncontrolled Keywords: |
Amino Acid Sequence
Base Sequence
Fungal Proteins genetics
GTP Phosphohydrolases metabolism
GTPase-Activating Proteins
Genetic Complementation Test
Genetic Vectors
Humans
Molecular Sequence Data
Neurofibromatosis 1 genetics
Neurofibromin 1
Oligonucleotide Probes
Oncogene Protein p21(ras) genetics
RAS
Plasmids
Proteins genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins
Sequence Homology, Nucleic Acid
ras GTPase-Activating Proteins |
| Subjects: |
diseases & disorders > congenital hereditary genetic diseases
diseases & disorders > nervous system diseases and disorders
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization |
| CSHL Authors: |
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| Communities: |
CSHL labs > Wigler lab |
| Depositing User: |
CSHL Librarian
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| Date: |
16 November 1990 |
| Date Deposited: |
19 Apr 2012 16:20 |
| Last Modified: |
18 Nov 2016 16:36 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26151 |
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