Thiagalingam, S., Lisitsyn, N. A., Hamaguchi, M., Wigler, M. H., Willson, J. K., Markowitz, S. D., Leach, F. S., Kinzler, K. W., Vogelstein, B. (July 1996) Evaluation of the FHIT gene in colorectal cancers. Cancer Res, 56 (13). pp. 2936-9. ISSN 0008-5472 (Print)
Abstract
A variety of studies suggests that tumor suppressor loci on chromosome 3p are important in various forms of human neoplasia. Recently, a chromosome 3p14.2 gene called FHIT was discovered and proposed as a candidate tumor suppressor gene in colorectal and other cancers. We evaluated the FHIT gene in a panel of colorectal cancer cell lines and xenografts, which allowed a comprehensive mutational analysis. A transcript containing the complete coding sequence was found to be expressed at robust levels in 29 of 31 cancers tested. The complete sequence of the coding region of the gene was determined and found to be normal in all 29 of these cases. These studies suggest either that FHIT is inactivated by an unusual mechanism or that it plays a role in relatively few colorectal tumors.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Acid Anhydride Hydrolases Alleles Base Sequence Chromosomes Human, Pair 3 Colorectal Neoplasms genetics DNA, Neoplasm genetics Evaluation Studies Gene Deletion Genes Tumor Suppressor Homozygote Humans Molecular Sequence Data Neoplasm Proteins genetics Polymerase Chain Reaction Proteins genetics Transcription Genetic Tumor Cells Cultured |
| Subjects: | diseases & disorders > cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > suppressor |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1 July 1996 |
| Date Deposited: | 06 Apr 2012 13:41 |
| Last Modified: | 04 Nov 2016 14:58 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26000 |
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