Van Aelst, L., Barr, M., Marcus, S., Polverino, A., Wigler, M. H. (July 1993) Complex formation between RAS and RAF and other protein kinases. Proc Natl Acad Sci U S A, 90 (13). pp. 6213-7. ISSN 0027-8424 (Print)
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Abstract
We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Fungal Proteins metabolism GTP-Binding Proteins metabolism Guanosine Triphosphate metabolism MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Protein Kinases metabolism Protein-Serine Threonine Kinases metabolism Protein Tyrosine Kinase metabolism Proto-Oncogene Proteins metabolism Proto-Oncogene Proteins c-raf Proto-Oncogene Proteins p21(ras) metabolism Saccharomyces cerevisiae metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces metabolism Schizosaccharomyces pombe Proteins ras Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Van Aelst lab CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1 July 1993 |
| Date Deposited: | 06 Apr 2012 13:56 |
| Last Modified: | 09 Nov 2017 19:59 |
| PMCID: | PMC46898 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25991 |
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