Transformation of mammalian cells with an amplifiable dominant acting gene

Wigler, M. H., Perucho, M., Kurtz, D., Dana, S., Pellicer, A., Axel, R., Silverstein, S. (June 1980) Transformation of mammalian cells with an amplifiable dominant acting gene. Proceedings of the National Academy of Sciences of the United States of America, 77 (6). pp. 3567-3570. ISSN 0027-8424

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We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have provided direct evidence for gene transfer. Transformants selected for increased resistance to methotrexate contain increased amounts of the newly transferred gene. We have used this mutant dhfr gene to introduce the Escherichia coli antibiotic resistance plasmid pBR322 into animal cells. Amplification of the dhfr sequences results in amplification of the pBR322 sequences as well. The use of this gene may allow the introduction and amplification of virtually any genetic element in various new cellular environments.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: June 1980
Date Deposited: 06 Apr 2012 18:30
Last Modified: 30 Sep 2019 19:49
PMCID: PMC349658
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