Wigler, M. H., Perucho, M., Kurtz, D., Dana, S., Pellicer, A., Axel, R., Silverstein, S. (June 1980) Transformation of mammalian cells with an amplifiable dominant acting gene. Proceedings of the National Academy of Sciences of the United States of America, 77 (6). pp. 3567-3570. ISSN 0027-8424
Preview |
PDF
Wigler_PNAS_1980transformation.pdf - Published Version Download (1MB) | Preview |
Abstract
We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have provided direct evidence for gene transfer. Transformants selected for increased resistance to methotrexate contain increased amounts of the newly transferred gene. We have used this mutant dhfr gene to introduce the Escherichia coli antibiotic resistance plasmid pBR322 into animal cells. Amplification of the dhfr sequences results in amplification of the pBR322 sequences as well. The use of this gene may allow the introduction and amplification of virtually any genetic element in various new cellular environments.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | June 1980 |
| Date Deposited: | 06 Apr 2012 18:30 |
| Last Modified: | 30 Sep 2019 19:49 |
| PMCID: | PMC349658 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25962 |
Actions (login required)
 |
Administrator's edit/view item |
