The adenylyl cyclase gene from Schizosaccharomyces pombe

Young, D., Riggs, M., Field, J., Vojtek, A., Broek, D., Wigler, M. H. (October 1989) The adenylyl cyclase gene from Schizosaccharomyces pombe. Proc Natl Acad Sci U S A, 86 (20). pp. 7989-93. ISSN 0027-8424

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Abstract

We cloned the adenylyl cyclase gene from the fission yeast Schizosaccharomyces pombe using low-stringency hybridization to the Saccharomyces cerevisiae adenylyl cyclase gene. The Sc. pombe gene encodes a 1692-amino acid-residue protein. The identity of this gene was confirmed by studies of its expression in Sa. cerevisiae. Expression of the carboxyl-terminal region of the Sc. pombe adenylyl cyclase protein will suppress a temperature-sensitive mutation in the Sa. cerevisiae adenylyl cyclase gene. Furthermore, Sa. cerevisiae that lack their endogenous adenylyl cyclase gene and express the carboxyl-terminal region of the Sc. pombe adenylyl cyclase protein have measurable adenylyl cyclase activity. The carboxyl-terminal region of this protein has strong homology with the catalytic domain of the Sa. cerevisiae adenylyl cyclase. Also, Sc. pombe adenylyl cyclase, like Sa. cerevisiae adenylyl cyclase, contains a tandemly repeated motif rich in leucine. Neither yeast protein is particularly homologous to the recently cloned Gs-responsive mammalian adenylyl cyclase [Krupinski, J., Coussen, F., Bakalyar, H. A., Tang, W.-J., Feinstein, P. G., Orth, K., Slaughter, C., Reed, R. R. & Gilman, A. G. (1989) Science 244, 1558-1564].

Item Type:	Paper
Uncontrolled Keywords:	Adenylate Cyclase genetics metabolism Amino Acid Sequence Base Sequence Cloning, Molecular Comparative Study Gene Expression Genes, Structural Fungal Molecular Sequence Data Plasmids Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae enzymology genetics Saccharomycetales genetics Schizosaccharomyces enzymology genetics Sequence Homology Nucleic Acid
Subjects:	organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering
CSHL Authors:	Wigler, Michael H.
Communities:	CSHL labs > Wigler lab
Depositing User:	CSHL Librarian
Date:	October 1989
Date Deposited:	10 Apr 2012 14:00
Last Modified:	09 Nov 2017 20:45
PMCID:	PMC298198
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/25943

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