Cancer modeling meets human organoid technology

Tuveson, D., Clevers, H. (June 2019) Cancer modeling meets human organoid technology. Science, 364 (6444). pp. 952-955. ISSN 0036-8075

Abstract

Organoids are microscopic self-organizing, three-dimensional structures that are grown from stem cells in vitro. They recapitulate many structural and functional aspects of their in vivo counterpart organs. This versatile technology has led to the development of many novel human cancer models. It is now possible to create indefinitely expanding organoids starting from tumor tissue of individuals suffering from a range of carcinomas. Alternatively, CRISPR-based gene modification allows the engineering of organoid models of cancer through the introduction of any combination of cancer gene alterations to normal organoids. When combined with immune cells and fibroblasts, tumor organoids become models for the cancer microenvironment enabling immune-oncology applications. Emerging evidence indicates that organoids can be used to accurately predict drug responses in a personalized treatment setting. Here, we review the current state and future prospects of the rapidly evolving tumor organoid field.

Item Type:	Paper
Subjects:	Investigative techniques and equipment > cell culture > cancer organoids Investigative techniques and equipment > CRISPR-Cas9 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:	Tuveson, David A.
Communities:	CSHL labs > Tuveson lab
Depositing User:	Matthew Dunn
Date:	7 June 2019
Date Deposited:	05 Aug 2019 16:12
Last Modified:	05 Aug 2019 16:12
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/38149

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