An atlas of chromatoid body components

Meikar, O., Vagin, V. V., Chalmel, F., Sostar, K., Lardenois, A., Hammell, M., Jin, Y., Da Ros, M., Wasik, K. A., Toppari, J., Hannon, G. J., Kotaja, N. (April 2014) An atlas of chromatoid body components. RNA, 20 (4). pp. 483-95. ISSN 1355-8382

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Abstract

The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a unique, unusually large cytoplasmic granule, the chromatoid body (CB), which emerges during the switch between the meiotic and post-meiotic phases of spermatogenesis. To better understand the role of the CB in male germ cell differentiation, we isolated CBs from mouse testes and revealed its full RNA and protein composition. We showed that the CB is mainly composed of RNA-binding proteins and other proteins involved RNA regulation. The CB was loaded with RNA, including pachytene piRNAs, a diverse set of mRNAs, and a number of uncharacterized long noncoding transcripts. The CB was demonstrated to accumulate nascent RNA during all the steps of round spermatid differentiation. Our results revealed the CB as a large germ cell-specific RNP platform that is involved in the control of the highly complex transcriptome of haploid male germ cells.

Item Type:	Paper
Uncontrolled Keywords:	chromatoid body male germ cells piRNA post-transcriptional ribonucleoprotein granule
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:	Hammell, Molly Hannon, Gregory J. Jin, Ying Vagin, Vasily V. Wasik, Kaja
Communities:	CSHL Cancer Center Program > Cancer Genetics CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL Cancer Center Shared Resources > Mass Spectrometry Service CSHL Cancer Center Shared Resources > Proteomics Service CSHL labs > Hammell M. lab CSHL labs > Hannon lab School of Biological Sciences > Publications CSHL Cancer Center Shared Resources > Bioinformatics Service
Depositing User:	Matt Covey
Date:	April 2014
Date Deposited:	11 Apr 2014 19:56
Last Modified:	09 Nov 2017 21:17
PMCID:	PMC3964910
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/29739

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