Maize R2R3 Myb genes: Sequence analysis reveals amplification in the higher plants

Rabinowicz, P. D., Braun, E. L., Wolfe, A. D., Bowen, B., Grotewold, E. (September 1999) Maize R2R3 Myb genes: Sequence analysis reveals amplification in the higher plants. Genetics, 153 (1). pp. 427-44. ISSN 0016-6731 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10471724

Abstract

Transcription factors containing the Myb-homologous DNA-binding domain are widely found in eukaryotes. In plants, R2R3 Myb-domain proteins are involved in the control of form and metabolism. The Arabidopsis genome harbors >100 R2R3 Myb genes, but few have been found in monocots, animals, and fungi. Using RT-PCR from different maize organs, we cloned 480 fragments corresponding to a 42-44 residue-long sequence spanning the region between the conserved DNA-recognition helices (Myb(BRH)) of R2R3 Myb domains. We determined that maize expresses >80 different R2R3 Myb genes, and evolutionary distances among maize Myb(BRH) sequences indicate that most of the amplification of the R2R3 Myb gene family occurred after the origin of land plants but prior to the separation of monocots and dicots. In addition, evidence is provided for the very recent duplication of particular classes of R2R3 Myb genes in the grasses. Together, these findings render a novel line of evidence for the amplification of the R2R3 Myb gene family in the early history of land plants and suggest that maize provides a possible model system to examine the hypothesis that the expansion of Myb genes is associated with the regulation of novel plant cellular functions.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Cloning, Molecular Codon/genetics Comparative Study DNA-Binding Proteins/chemistry/ genetics Evolution, Molecular Gene Amplification/ genetics Gene Duplication Genes, Plant/ genetics/physiology Humans Molecular Sequence Data Multigene Family/genetics Mutation/genetics Phylogeny Plant Components/genetics Plant Proteins/chemistry/ genetics Proto-Oncogene Proteins c-myb Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Sequence Alignment Variation (Genetics)/genetics Zea mays/ genetics
Subjects: organism description > plant > Arabidopsis
organism description > plant > maize
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene amplification
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Kathleen Darby
Date: September 1999
Date Deposited: 23 Apr 2014 17:00
Last Modified: 30 Apr 2014 17:20
PMCID: PMC1460732
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29837

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