Duplication and suppression of chloroplast protein translocation genes in maize

Settles, A. M., Baron, A., Barkan, A., Martienssen, R. A. (January 2001) Duplication and suppression of chloroplast protein translocation genes in maize. Genetics, 157 (1). pp. 349-360. ISSN 0016-6731

URL: http://www.ncbi.nlm.nih.gov/pubmed/11139515

Abstract

The HCF106 (high chlorophyll flourescence) gene of maize encodes a chloroplast membrane protein required for tr,translocation of a subset of proteins across the thylakoid membrane. Mutations in HCF106 caused by the insertion of Robertson's Mutator transposable elements have been mapped to chromosome 2S. Here we show that there is a closely related homolog of HCF106 encoded elsewhere in the maize genome (HCF106c) that can partially compensate fur these mutations. This homolog maps on chromosome 10L and is part of the most recent set of segmental duplications in the maize genome. Triple mutants that are disrupted in both the HCF106 and Sec-dependent protein translocation pathways provide evidence that the act independently. The HCF106c gene accounts for a previously reported exception to the correlation between epigenetic suppression of hcf106 and methylation of Mutator transposons. We also demonstrate that insertions of Robertson's Mutator elements into either introns or promoters can lead to mutations whose phenotypes are suppressed in the absence of Mu activity, while alleles with insertions in both positions are not suppressed. The implications of these observations are discussed.

Item Type: Paper
Uncontrolled Keywords: transposable element thylakoid membrane molecular-cloning escherichia-coli dna modification seca homolog zea-mays mutator pathways expression
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA translocation
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: January 2001
Date Deposited: 13 Dec 2013 15:15
Last Modified: 13 Dec 2013 15:15
PMCID: PMC1461481
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29000

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