RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Zaratiegui, M., Castel, S. E., Irvine, D. V., Kloc, A., Ren, J., Li, F., de Castro, E., Marin, L., Chang, A. Y., Goto, D., Cande, W. Z., Antequera, F., Arcangioli, B., Martienssen, R. A. (November 2011) RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. Nature, 479 (7371). pp. 135-8. ISSN 1476-4687 (Electronic)0028-0836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/22002604
DOI: 10.1038/nature10501

Abstract

Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

Item Type: Paper
Uncontrolled Keywords: Centromere genetics metabolism Chromosomal Proteins Non-Histone genetics metabolism DNA Damage DNA Replication physiology DNA-Directed DNA Polymerase metabolism Gene Silencing Heterochromatin genetics metabolism Histones metabolism Homologous Recombination Models Genetic Molecular Sequence Data RNA Interference RNA Polymerase II metabolism RNA, Small Interfering genetics metabolism Replication Origin S Phase Schizosaccharomyces genetics Schizosaccharomyces pombe Proteins genetics metabolism Transcription Genetic
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
CSHL Authors:
Communities: CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Antibody and Phage Display Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL Post Doctoral Fellows
CSHL labs > Martienssen lab
Watson School > Publications
CSHL Cancer Center Shared Resources > Mass Spectrometry Service
Depositing User: Matt Covey
Date: 3 November 2011
Date Deposited: 06 Feb 2013 21:40
Last Modified: 30 Oct 2015 14:42
PMCID: PMC3391703
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27164

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