Zaratiegui, M., Castel, S. E., Irvine, D. V., Kloc, A., Ren, J., Li, F., de Castro, E., Marin, L., Chang, A. Y., Goto, D., Cande, W. Z., Antequera, F., Arcangioli, B., Martienssen, R. A.
(November 2011)
RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II.
Nature, 479 (7371).
pp. 135-8.
ISSN 1476-4687 (Electronic)0028-0836 (Linking)
Abstract
Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Centromere genetics metabolism
Chromosomal Proteins Non-Histone genetics metabolism
DNA Damage
DNA Replication physiology
DNA-Directed DNA Polymerase metabolism
Gene Silencing
Heterochromatin genetics metabolism
Histones metabolism
Homologous Recombination
Models Genetic
Molecular Sequence Data
RNA Interference
RNA Polymerase II metabolism
RNA, Small Interfering genetics metabolism
Replication Origin
S Phase
Schizosaccharomyces genetics
Schizosaccharomyces pombe Proteins genetics metabolism
Transcription Genetic |
| Subjects: |
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi |
| CSHL Authors: |
|
| Communities: |
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Antibody and Phage Display Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL Post Doctoral Fellows
CSHL labs > Martienssen lab
School of Biological Sciences > Publications
CSHL Cancer Center Shared Resources > Mass Spectrometry Service |
| Depositing User: |
Matt Covey
|
| Date: |
3 November 2011 |
| Date Deposited: |
06 Feb 2013 21:40 |
| Last Modified: |
30 Oct 2015 14:42 |
| PMCID: |
PMC3391703 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27164 |
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