Stimulation of membrane ruffling and MAP kinase activation by distinct effectors of RAS

Joneson, T., White, M. A., Wigler, M. H., Bar-Sagi, D. (February 1996) Stimulation of membrane ruffling and MAP kinase activation by distinct effectors of RAS. Science, 271 (5250). pp. 810-812. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/8628998
DOI: 10.1126/science.271.5250.810

Abstract

The RAS guanine nucleotide binding proteins activate multiple signaling events that regulate cell growth and differentiation. In quiescent fibroblasts, ectopic expression of activated H-RAS (H-RAS(V12), where V12 indicates valine-12) induces membrane ruffling, mitogen-activated protein (MAP) kinase activation, and stimulation of DNA synthesis. A mutant of activated H-RAS, H-RAS(V12C40) (where C40 indicates cysteine-40), was identified that was defective for MAP kinase activation and stimulation of DNA synthesis, but retained the ability to induce membrane ruffling. Another mutant of activated H-RAS, H-RAS(V12S35) (where S35 indicates serine-35), which activates MAP kinase, was defective for stimulation of membrane ruffling and induction of DNA synthesis. Expression of both mutants resulted in a stimulation of DNA synthesis that was comparable to that induced by H-RAS(V12). These results indicate that membrane ruffling and activation of MAP kinase represent distinct RAS effector pathways and that input from both pathways is required for the mitogenic activity of RAS.

Item Type: Paper
Uncontrolled Keywords: SIGNAL-TRANSDUCTION signal transduction PROTEIN
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: February 1996
Date Deposited: 12 Apr 2012 16:47
Last Modified: 02 Nov 2016 16:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26202

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