Sequence and Structure of the Coding Regions of the Human H-Ras-1 Gene from T-24 Bladder Carcinoma Cells

Fasano, O., Taparowsky, E., Fiddes, J., Wigler, M. H., Goldfarb, M. (1983) Sequence and Structure of the Coding Regions of the Human H-Ras-1 Gene from T-24 Bladder Carcinoma Cells. Journal of Molecular & Applied Genetics, 2 (2). pp. 173-180. ISSN 0271-6801

URL: https://www.ncbi.nlm.nih.gov/pubmed/6308118

Abstract

c[complementary]DNA was molecularly cloned and sequenced to the transcript of H-ras-1, the transforming gene of the T24 human bladder carcinoma cell line. The transcript derives from at least 5 exons in the H-ras-1 gene, and RNA splicing occurs at sites typical of exon-intron junctions. T24 H-ras-1 RNA has an AUG-initiated open reading frame of 567 nucleotides, which can encode a protein of mass comparable to the apparent MW of the T24 H-ras-1 gene product. The T24 H-ras-1 gene product is nearly identical to v-H-ras p21, the transforming protein encoded by the genome of Harvey sarcoma virus. The implications of this sequence conservation in the structure-function relationships of ras proteins are discussed.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
diseases & disorders > cancer > cancer types > bladder cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1983
Date Deposited: 13 Apr 2012 20:54
Last Modified: 24 Feb 2017 21:56
URI: http://repository.cshl.edu/id/eprint/26178

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