Fasano, O., Taparowsky, E., Fiddes, J., Wigler, M. H., Goldfarb, M. (1983) Sequence and Structure of the Coding Regions of the Human H-Ras-1 Gene from T-24 Bladder Carcinoma Cells. Journal of Molecular & Applied Genetics, 2 (2). pp. 173-180. ISSN 0271-6801
Abstract
c[complementary]DNA was molecularly cloned and sequenced to the transcript of H-ras-1, the transforming gene of the T24 human bladder carcinoma cell line. The transcript derives from at least 5 exons in the H-ras-1 gene, and RNA splicing occurs at sites typical of exon-intron junctions. T24 H-ras-1 RNA has an AUG-initiated open reading frame of 567 nucleotides, which can encode a protein of mass comparable to the apparent MW of the T24 H-ras-1 gene product. The T24 H-ras-1 gene product is nearly identical to v-H-ras p21, the transforming protein encoded by the genome of Harvey sarcoma virus. The implications of this sequence conservation in the structure-function relationships of ras proteins are discussed.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS diseases & disorders > cancer > cancer types > bladder cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1983 |
| Date Deposited: | 13 Apr 2012 20:54 |
| Last Modified: | 24 Feb 2017 21:56 |
| URI: | https://repository.cshl.edu/id/eprint/26178 |
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