The Ras Oncoprotein and M-Phase Activity

Daar, I., Nebreda, A. R., Yew, N., Sass, P., Paules, R., Santos, E., Wigler, M. H., Vandewoude, G. F. (July 1991) The Ras Oncoprotein and M-Phase Activity. Science, 253 (5015). pp. 74-76. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/1829549
DOI: 10.1126/science.1829549

Abstract

The endogenous mos proto-oncogene product (Mos) is required for meiotic maturation In Xenopus oocytes, the ras oncogene product (Ras) can induce meiotic maturation and high levels of M-phase-promoting factor (MPF) independent of endogenous Mos, indicating that a parallel pathway to metaphase exists. In addition, Ras, like Mos and cytostatic factor, can arrest Xenopus embryonic cell cleavage in mitosis and maintain high levels of MPF. Thus, in the Xenopus oocyte and embryo systems Ras functions in the M phase of the cell cycle. The embryonic cleavage arrest assay is a rapid and sensitive test for Ras function.

Item Type: Paper
Uncontrolled Keywords: MOS PROTO-ONCOGENE MOS proto-oncogene MATURATION-PROMOTING FACTOR maturation promoting factor MURINE SARCOMA-VIRUS murine sarcoma virus XENOPUS OOCYTES Xenopus oocytes CELL-CYCLE cell cycle MEIOTIC MATURATION meitotic maturation GENE-PRODUCT gene product ARREST arrest P21 p21 EGGS eggs
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > animal > developmental stage > embryo
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: July 1991
Date Deposited: 19 Apr 2012 14:38
Last Modified: 18 Nov 2016 17:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26174

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