Cooperative interaction of S. pombe proteins required for mating and morphogenesis

Chang, E. C., Barr, M., Wang, Y., Jung, V., Xu, H. P., Wigler, M. H. (October 1994) Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell, 79 (1). pp. 131-41. ISSN 0092-8674 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/7923372
DOI: 10.1016/0092-8674(94)90406-5

Abstract

We isolated two S. pombe genes, scd1 and scd2, that are required for normal morphology and mating. scd1 and scd2 are homologous to CDC24 and BEM1, respectively, of S. cerevisiae. Epistasis analyses indicate that scd2 and ras1 converge upon scd1, which, in turn, interacts with cdc42sp, a RHO-like GTPase. Studies with the yeast two-hybrid system indicate that scd2 forms complexes with both scd1 and cdc42sp. Furthermore, biochemical studies indicate that the interaction between scd1 and scd2 is direct. The yeast two-hybrid data further suggest that scd1, scd2, cdc42sp, and ras1, in its GTP-bound state, act cooperatively to form a protein complex.

Item Type: Paper
Uncontrolled Keywords: Adaptor Proteins Signal Transducing Amino Acid Sequence Base Sequence Cell Cycle Proteins genetics metabolism Cloning Molecular Epistasis Genetic Fungal Proteins genetics/metabolism GTP-Binding Proteins genetics/metabolism Genes Fungal genetics Genetic Complementation Test Guanine Nucleotide Exchange Factors Models Genetic Molecular Sequence Data Morphogenesis Proto-Oncogene Proteins genetics/metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces genetics/metabolism/physiology Schizosaccharomyces pombe Proteins Sequence Analysis DNA Sequence Deletion physiology Sequence Homology Amino Acid cdc42 GTP-Binding Protein Saccharomyces cerevisiae ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTPase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 7 October 1994
Date Deposited: 19 Apr 2012 14:11
Last Modified: 18 Nov 2016 16:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26169

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