Structure and Activation of the Human N-Ras Gene

Taparowsky, E., Shimizu, K., Goldfarb, M., Wigler, M. H. (1983) Structure and Activation of the Human N-Ras Gene. Cell, 34 (2). pp. 581-586.

URL: https://www.ncbi.nlm.nih.gov/pubmed/6616621
DOI: 10.1016/0092-8674(83)90390-2

Abstract

The normal human N-ras gene was cloned. In structure and sequence it closely resembles the human H-ras and K-ras genes. The 3 genes share regions of nucleotide homology and nucleotide divergence within coding sequences and have a common intron/exon structure, indicating that they evolved from a similarly spliced ancestral gene. The N-ras gene of SK-N-SH neuroblastoma cells has transforming activity, while the normal N-ras gene does not, the result of a single nucleotide change substituting Lys for Gln in position 61 of the N-ras gene product. Amino acid substitutions in 2 distinct regions can activate the transforming potential of ras gene products.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > annotation > sequence annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1983
Date Deposited: 06 Apr 2012 13:48
Last Modified: 18 Nov 2016 16:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26003

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