Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex

Enemark, E. J., Stenlund, A., Joshua-Tor, L. (2002) Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex. Embo Journal, 21 (6). pp. 1487-1496. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/11889054
DOI: 10.1093/emboj/21.6.1487

Abstract

Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus > papillomavirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
CSHL labs > Stenlund lab
Depositing User: CSHL Librarian
Date Deposited: 21 Mar 2012 20:01
Last Modified: 05 Sep 2017 18:58
PMCID: PMC125917
Related URLs:
Dataset ID:
URI: http://repository.cshl.edu/id/eprint/25491

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving