Enemark, E. J., Stenlund, A., Joshua-Tor, L. (March 2002) Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex. Embo Journal, 21 (6). pp. 1487-1496. ISSN 0261-4189 (Public Dataset)
Abstract
Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organism description > virus > papillomavirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs > Joshua-Tor lab CSHL labs > Stenlund lab |
| Depositing User: | CSHL Librarian |
| Date: | March 2002 |
| Date Deposited: | 21 Mar 2012 20:01 |
| Last Modified: | 05 Sep 2017 18:58 |
| PMCID: | PMC125917 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/25491 |
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